Comparative evaluation of pH and Ca+ ion release from MTA on interaction with platelet-rich fibrin and blood clot: an in vitro study

Background: ‘Regenerative endodontics’ using host-derived scaffolds and biomaterials (MTA) is popular in the management of teeth with open apex. Alkaline pH bioactivity contribute to tissue healing remineralization. We assessed influence PRF Blood Clot on Ca+ ion release from MTA. Methods: A total 15 single-rooted human extracted were sectioned at level cementoenamel junction. Based type used, samples divided into three groups. Group 1 (MTA+ PRF), 2 (MTA + Clot), 3 (control MTA). The prepared specimens transferred a fresh falcon tube containing 10mL distilled water collected solutions analysed for 3h, seventh day 14th day. Results: It was observed that mean significantly lower experimental groups as compared control group. Though there an increase recorded all time periods, difference not significant. peaked Day 7 (Group3 > Group2 Group1) reduced groups. Conclusions: Within limitations study, it can be concluded blood clot Ca+ ion MTA.